A US military MQ-9 drone was shot down in Yemen's Dhamar region, southeast of the Houthi-controlled capital Sanaa, two US officials told Reuters on Wednesday, the second such incident in recent months.
A Houthi military spokesman had earlier said that air defenses had brought down a US drone.
The officials, speaking on the condition of anonymity, said the drone was shot down late on Tuesday.
This is not the first time a US drone has been shot down in Yemen. In June, the US military said that Houthi rebels had shot down a US government-operated drone with assistance from Iran.
US forces have occasionally launched drone and air strikes against Yemen’s al-Qaeda branch, known as Al-Qaeda in the Arabian Peninsula (AQAP).
One of the officials said that it appeared that the armed military drone had been shot down by a surface-to-air missile operated by the Iran-aligned Houthi group.
"It appears to have been fired by the Houthis and enabled by Iran," the official said, without providing details or specific evidence.
The official said that while losing a drone was expensive, it was not unprecedented and it was unlikely to lead to any major response by the United States.
The other official cautioned that it was too early to tell who was responsible for the incident.
Iran rejects accusations from the United States and its Gulf Arab allies that Tehran is providing military and financial support to the Houthis and blames Riyadh for the deepening crisis there.
Overnight, Houthi military spokesman Yahya Saria said that the drone had been shot down.
"The rocket which hit it was developed locally and will be revealed soon at a press conference," Saria said on Twitter.
"Our skies are no longer open to violations as they once were and the coming days will see great surprises," he added.
The drone shoot-down comes as tensions between Iran and the United States have risen since President Donald Trump's administration last year quit an international deal to curb Iran's nuclear ambitions and began to ratchet up sanctions. Iranian officials denounced the new penalties as "economic warfare."
Iranian Foreign Minister Mohammad Javad Zarif said on Wednesday Tehran may act "unpredictably" in response to the United States' "unpredictable" policies under Trump.